Direct Reprobing with Anti-β-actin Antibody as an Internal Control for Western Blotting Analysis
نویسندگان
چکیده
منابع مشابه
Direct reprobing with anti-beta-actin antibody as an internal control for western blotting analysis.
Western blotting analysis is the most common method for identification and quantification of a specific protein in complex protein mixtures (2–4). To compare different levels of an interesting protein derived from different sources, such as from different tissues or from cell cultures exposed to a variety of treatments, an internal control is necessary to ensure even sample loading and gel tran...
متن کاملTotal Protein Analysis as a Reliable Loading Control for Quantitative Fluorescent Western Blotting
Western blotting has been a key technique for determining the relative expression of proteins within complex biological samples since the first publications in 1979. Recent developments in sensitive fluorescent labels, with truly quantifiable linear ranges and greater limits of detection, have allowed biologists to probe tissue specific pathways and processes with higher resolution than ever be...
متن کاملProduction and characterization of polyclonal antibody against a synthetic peptide from β-actin protein
Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. The aim of this study was to produce polyclonal antibody against a synthetic peptide derived from N-terminal region of β-actin protein to be used as a protein loading control in western ...
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Histological Analysis and Immunohistochemistry Renal sections (3 μm thick) were stained with periodic acid-Schiff (PAS) and examined under light microscopy. Glomerulosclerosis index was semiquantitatively graded as 0 to 4+; vascular injury score as 0 to 3+; and tubulointerstitial injury score as 0 to 5+, according to the criteria reported previously. Ultrastructure of glomerular podocytes was a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00282bm05